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Bitcoin Roars Back Again, Has The Recovery Began or Just another Fake Pump? 2021

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2022.01.25 04:20 TrendsWide Bitcoin Roars Back Again, Has The Recovery Began or Just another Fake Pump? 2021

Bitcoin Roars Back Again, Has The Recovery Began or Just another Fake Pump? 2021 submitted by TrendsWide to TrendsNewsWorld [link] [comments]


2022.01.25 04:20 schowdur123 CHO DHFR- CELLS and selection by methotrexate

Hello fellow labrats,
We have some cho dhfr minus cells. They are growing in kaighns f-12 media plus serum plus hat supplement. They seem to be doing fine in it.
We transfected these cells by a plasmid containing the gene of interest along with dhfr. The cells are still alive and doing fine.
We want to select these cells and since this is my first attempt, I was told to use methotrexate. The principle apparently is that mtx will inhibit dhfr and only the cells consuming enough copies of dhfr will survive and thrive.
I wanted to ask the experts three questions. 1. In the selection media, would you withdraw the hat supplement? 2. Can you use kaighn's f-12 along with serum still as your base media? I know addition of glutamine is recommended. 3. What ballpark concentration of mtx do you recommend to get started?
Thank you everyone for your guidance!
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2022.01.25 04:20 thanosdummythick69 fun/weird cars in fh5?

hey i was just wanting to find more fun cars or weird cars or cars with weird tunes or something similar, thanks
submitted by thanosdummythick69 to ForzaHorizon [link] [comments]


2022.01.25 04:20 Suspicious-Singer164 Anyone on this thread happen to have this snowboard they’d be willing to sell/ship if need be. Been looking for it since 2019. Size preferably would be around the 157cm but if it’s the board I’m not afraid to ride a short board because she is my dream board. (Name of the board in pic)

Anyone on this thread happen to have this snowboard they’d be willing to sell/ship if need be. Been looking for it since 2019. Size preferably would be around the 157cm but if it’s the board I’m not afraid to ride a short board because she is my dream board. (Name of the board in pic) submitted by Suspicious-Singer164 to snowboarding [link] [comments]


2022.01.25 04:20 wise_european Thank you, Mr. Rothschild, very based

Thank you, Mr. Rothschild, very based submitted by wise_european to MadeMeSmile [link] [comments]


2022.01.25 04:20 NewsElfForEnterprise ‘Brutal,’ ‘crazy’ housing market has Seattle-area homes selling half-million over asking price

‘Brutal,’ ‘crazy’ housing market has Seattle-area homes selling half-million over asking price submitted by NewsElfForEnterprise to News_RealEstate [link] [comments]


2022.01.25 04:20 TrendsWide CHRISTOPHER HART: Why ARE the custodians of our culture so ashamed of it?

CHRISTOPHER HART: Why ARE the custodians of our culture so ashamed of it? submitted by TrendsWide to TrendsNewsWorld [link] [comments]


2022.01.25 04:20 ockehn This is a complete embarrassment. This team is going nowhere without some changes. What are they doing?

This is a complete embarrassment. This team is going nowhere without some changes. What are they doing? submitted by ockehn to caps [link] [comments]


2022.01.25 04:20 Available_Pin9190 ScorpionFinance - $SCORPFIN | 10% Reflection Reward In $BNB | 2% Auto Buyback & Burn ScorpFin | 5% Reflection Reward In ScorpFin | Hyper-Deflationionary mechanism | 3% Auto Growing Liquidity | Whale Proof | Anti Bot and Dump | Audit by Interfi

ScorpFin encompasses the qualities of a secure self-hosted wallet, a DEX, a merchant payment network, Gaming, and a Defi farming and staking services aggregator within a single application.
5 ScorpFin DApps
ScorpFolio - this product aims to be the leading network for mobile cryptocurrency portfolio tracking management, where users can overview and customize their wallet assets portfolio.
ScorPay - is a universal mobile app that allows users to buy goods and services using cryptocurrencies.
ScorpSwap - this product is a completely different type of exchange that's fully decentralized - meaning it is owned and operated by a single entity and uses a relatively new type of trading model called an automated liquidity protocol. This platform is built on the BSC network.
ScorpNFTracker & Market - this product represents a marketplace for buying, selling, and creating NFTs. It uses sales data of each NFT, thus, in turn, rewarding users with ScoprFin tokens.
ScorpGames - is a new type of game partially owned and operated by its Players. By owning ScorpNFTs, players have more chances of completing the stages and ultimately earn more rewards attached to each stage.
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2022.01.25 04:20 unoriginalgh i agree

i agree submitted by unoriginalgh to PurrfectTale [link] [comments]


2022.01.25 04:20 _vRay_ Nice Club

Nice Club submitted by _vRay_ to CoachCorySubmissions [link] [comments]


2022.01.25 04:20 Free-_-thinker Tell me which house your sun is in, what your venus sign is and in which house, and how you deal with heartbreak

Scorpio Sun and venus both 5th house and I‘m that dramatic bitch who starts crying every few hours (I usually never cry), throws the stuff which reminds me of them through my room, and overall just doesn‘t take it well (so you‘re also free to give me advice :,))
submitted by Free-_-thinker to Scorpio [link] [comments]


2022.01.25 04:20 AnonymousLoser70100 Mandolin before brunoise?

I’ve got to make a batch of bolognese today so big it would make an Italian grandmother proud. Issue is I don’t really have any tools to help speed this up other than a benriner mandolin and a laser knife. I was thinking I could use that to julienne the carrot and celery and then brunoise from there. Thoughts? Ordinarily I’d just to try it but I don’t want to risk wasting too much time on the clock.
submitted by AnonymousLoser70100 to KitchenConfidential [link] [comments]


2022.01.25 04:20 TrendsWide A recent study: Our brains keep us alive and protect us from surrounding dangers, even while we sleep | Sciences

A recent study: Our brains keep us alive and protect us from surrounding dangers, even while we sleep | Sciences submitted by TrendsWide to TrendsNewsWorld [link] [comments]


2022.01.25 04:20 Flashy-Respect-2201 Advice Needed

I am 15 years old and mastrubated alot since last 2-3 years and now I think that I have low cheekbones but my brother have high. so will my cheekbones also turn out to be high if I quit mastrubation? [as of now they are 1.5-2 cm below my eyes]
submitted by Flashy-Respect-2201 to nofapbrasil [link] [comments]


2022.01.25 04:20 lss_mobile_mod_02 Test link

submitted by lss_mobile_mod_02 to LssMobTest [link] [comments]


2022.01.25 04:20 qlfang Google Accelerates AR Headset Development: Report

Google Accelerates AR Headset Development: Report submitted by qlfang to MVIS [link] [comments]


2022.01.25 04:20 DonaviNata ART Donavi#5765

https://twitter.com/DonaviNat/status/1485874760504516608
submitted by DonaviNata to MoonbeamAmbassadors [link] [comments]


2022.01.25 04:20 swagNextTuber Florida man arrested at Waffle House after alleged outburst over bacon: report

Florida man arrested at Waffle House after alleged outburst over bacon: report submitted by swagNextTuber to TrendingQuickTVnews [link] [comments]


2022.01.25 04:20 Potential-Exit-438 Gamestop was 100% off today

submitted by Potential-Exit-438 to GME [link] [comments]


2022.01.25 04:20 TrendsWide Sarah Palin’s New York Times Lawsuit Is Delayed Because She Has COVID

submitted by TrendsWide to TrendsNewsWorld [link] [comments]


2022.01.25 04:20 TheAsAndYsReddit I’m Shrek. Ask me anything!

I’m Shrek from the movie “Shrek”. AMA!
submitted by TheAsAndYsReddit to AMA [link] [comments]


2022.01.25 04:20 archtech88 [WP]You've been 'friends' with a person for a long time. It was fine at first, but recently they've mocked you in front of your 'mutual friends' and treated you more like a servant than an equal. Now you're graduating, going to a college far away, and leaving them forever. They're not taking it well

submitted by archtech88 to WritingPrompts [link] [comments]


2022.01.25 04:20 iammathboy Do normally distributed residuals necessarily imply a normally distributed response?

This might be a trite question, but I'm struggling to come to a definitive answer to this that is based in theory or even supported by a set of good practical examples.
In linear regression:
Do normally distributed residuals necessarily imply a normally distributed response variable?
Relatedly, does a normally distributed response imply that residuals will also be normally distributed?
Looking at various sources and trying to generate some examples myself, I'm inclined to say, "No" in the first case but "yes" in the second case. I can't really articulate why, though.
(Not a homework problem, but rather something I was asked while tutoring.)
submitted by iammathboy to AskStatistics [link] [comments]


2022.01.25 04:20 swagNextTuber Mike Huckabee: Here’s why ‘Saturday Night Live’ is not funny

Mike Huckabee: Here’s why ‘Saturday Night Live’ is not funny submitted by swagNextTuber to TrendingQuickTVnews [link] [comments]


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